Effect of the brachypod mutation on cell adhesion and chondrogenesis in aggregates of mouse limb mesenchyme
نویسندگان
چکیده
Twelve-day normal and brachypod mouse limb mesenchyme was studied in rotation culture. Over a 3£ h period the rate of decline of single cells was significantly greater in mutant than in normal cultures, probably because the brachypod cells were more adhesive. However, the final size of the aggregates and their cell densities were the same by 24 h of incubation. On the other hand their pattern of chondrogenesis was different. Normal aggregates contained condensations with typical histotypic cartilage by 24 h of incubation, and were entirely chondrifled by 4 days in culture. The condensations in brachypod aggregates were fewer, smaller, and delayed in their chondrogenesis. Never more than 50% of the brachypod aggregate exhibited chondrogenesis. The importance of cell contact and cell density to the chondrogenic process are discussed.
منابع مشابه
Cell adhesion and chondrogenesis in brachypod mouse limb mesenchyme: fragment fusion studies.
This study is a continuing investigation of the effect of the brachypod mouse mutation on cell interactions and chondrogenesis during early limb development. In this report, cell adhesiveness was assessed in fused fragments of brachypod and normal limb-bud mesenchyme. Examination of the interface of fused distal postaxial limb fragments show brachypod limb mesenchyme to be more adhesive than no...
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